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New approaches for voltammetric determination of tumour biomarkers and antidotes in urine
Hrdlička, Vojtěch ; Barek, Jiří (advisor) ; Novotný, Ladislav (referee) ; Skopalová, Jana (referee)
This Ph. D. thesis presents new methods for the determination of selected clinically relevant electrochemically active compounds. The first part deals with development of determination of tumour biomarkers homovanillic acid (HVA) and vanillylmandelic acid (VMA) in human urine with the use of hollow-fibre based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at cathodically pre-treated boron doped diamond electrode (BDDE). Optimum conditions for HF-LPME-DPV of HVA and VMA were as follows: butyl benzoate as supported liquid membrane formed on porous polypropylene hollow-fibre, 0.1 mol L−1 HCl as donor phase and 30 min extraction time. Optimum acceptor phases were 0.1 mol L−1 phosphate buffer of pH 6 with ionic strength set to 0.55 mol L−1 for HVAand 0.1 mol L−1 NaOH for VMA, respectively. HF-LPME-DPV concentration dependencies for HVA and VMAwere linear in the range from 0.4 to 100 µmol L−1 and 0.5 to 100 µmol L−1 . Limits of quantification (LOQ)/detection (LOD) were 1.2/0.4 µmol L−1 for HVA and 1.7/0.5 µmol L−1 for VMA, respectively. The applicability of the developed methods was verified by analysis of human urine. In the second part, voltammetric behaviour of heavy metal poisoning antidote 2,3- dimercapto-1-propane-sulfonic acid (DMPS) was investigated with the use...

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